Development of Accessory Cells in B-Cell Compartments Is Retarted in B-Cell-Depleted Fetal Sheep

Development of Accessory Cells in B-Cell Compartments Is Retarted in B-Cell-Depleted Fetal Sheep

Accessory-cell populations in the lymphoid tissues of fetal sheep were investigated following depletion of B cells. An intraperitoneal injection of an anti-IgM antibody early in gestation resulted in a marked depletion of IgM+ cells in lymphoid tissues. Immune and enzyme histochemical techniques were used to identify accessory-cell populations in the ileal Peyer's patch, spleen, and lymph nodes...

In vitro Induction of Fetal Hemoglobin in Erythroid Cells Derived from CD133 Cells by Transforming Growth Factor-b and Stem Cell Factor

Increased fetal hemoglobin (HbF) in b-globin gene disorders ameliorates the clinical symptoms of the underlying disease. 5-azacytidine, butyrate and hydroxyurea, have been shown to activate g-globin gene expression. It has also been found that hematopoietic growth factors can influence expression of g-globin in erythroid cultures and in animal models. This study was designed to evaluate the in ...

In Vitro Cell Cycle Synchronization of Sheep Granulosa Cells

Purpose: To investigate the effect of different methods of synchronization on sheep granulosa cell cycle. Materials and Methods: Granulosa cells were aspirated from ovarian follicles and plated in a DMEM medium containing 15% FBS. Upon 70-80% confluency, the medium of the primarycultured as well as the passaged-5 cells were replaced with the medium containing either 0.5% FBS for 24, 48 and 72 h...

Glucocorticoids impair fetal b-cell development in rats

Blondeau, B., J. Lesage, P. Czernichow, J. P. Dupouy, and B. Bréant. Glucocorticoids impair fetal b-cell development in rats. Am J Physiol Endocrinol Metab 281: E592–E599, 2001.—In rats, poor fetal growth due to maternal food restriction during pregnancy is associated with decreased b-cell mass at birth and glucose intolerance in adulthood. Overexposure to glucocorticoids in utero can induce in...

Patterns of Gelatinase-B Expression in Leukemic Cell Lines